Journal: Journal of Cellular and Molecular Medicine

Article Title: Loganetin and 5‐fluorouracil synergistically inhibit the carcinogenesis of gastric cancer cells via down‐regulation of the Wnt/β‐catenin pathway

doi: 10.1111/jcmm.15932

Figure Lengend Snippet: 5FU and loganetin combine to cause cytotoxicity in HGC27 and MGC803 cells. (A) The molecular formula of loganetin. (B) The relative inhibition rate of HGC27 and MGC803 cells treated with loganin and loganetin (70μM) for 48 hours. (C and D) HGC27 and MGC803 cells were treated using the indicated doses of 5FU, loganetin, or a combination of the two for 48 hours, and survival was assessed via CCK8 assay. (E) HGC27 and MGC803 cells were exposed to 5FU (1.11 μM/ 1.25 μM), loganetin (40 μM), or both for 15 days, after which colony formation was assessed via crystal violet staining. A representative colony is on the left, while colony formation rates are on the right. (F) HGC27 and MGC803 cells were treated with 5FU (3.33 μM/ 2.5 μM), loganetin (70 μM), or both for 48 hours, and Annexin V/PI staining was used to assess apoptosis via flow cytometry. All statistical results were repeated for three times. * P <0.05; ** P <0.01; *** P <0.001

Article Snippet: To measure apoptosis, HGC27 or MGC803 cells were treated with 5FU, loganetin or both for 24 hours, followed by analysing with an Annexin V‐FITC/PI Apoptosis Detection Kit (Multi Sciences) based on the provided instructions.

Techniques: Inhibition, CCK-8 Assay, Staining, Flow Cytometry